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columbia blood agar plates  (ATCC)


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    Structured Review

    ATCC columbia blood agar plates
    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on <t>Columbia</t> <t>blood</t> <t>agar</t> <t>plates</t> after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.
    Columbia Blood Agar Plates, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A tumor-resident Staphylococcus epidermidis strain exhibits genomic and metabolic traits driving hepatocellular carcinoma progression"

    Article Title: A tumor-resident Staphylococcus epidermidis strain exhibits genomic and metabolic traits driving hepatocellular carcinoma progression

    Journal: iScience

    doi: 10.1016/j.isci.2026.116084

    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.
    Figure Legend Snippet: Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.

    Techniques Used: Isolation, Sequencing, Mass Spectrometry, Staining, Comparison, Incubation, Cell Culture, Inhibition

    Related Articles

    Inhibition:

    Article Title: Novel Nano Drug-Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI.
    Article Snippet: The OMV nanoparticle had a higher action on E.coli GIM 1.457 compared with the ATCC 25 922 strain, and so the onset time at 0–6 h of inhibition was obviously shorter. .. The inhibition zone diameters of ATCC 25 922 agar plates coated with NP-AM@OMV-P/H and NP-AM/FK@OMV-P/H coatings were 13.5 ± 0.3, 15.0 ± 0.3, and GIM1.457 agar plates were 14.0 ± 0.8, 16.5 ± 0.5 mm, respectively (Figure 3C). ..

    Article Title: Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
    Article Snippet: OMV drug‐loaded nanoparticles were more enriched on the dominant strain E.coli GIM1.457 compared with the ATCC 25 922 strain (Figure ), presumably owing to the sensitivity of EcN OMVs to the related bacterial strain. .. As shown in Figure 3 A , the inhibition zone diameter of the NP‐AM@OMV‐P/H coating on ATCC 25 922 agar plates was 19.5 ± 0.5 mm, and that on GIM1.457 agar plates was 23.7 ± 1.5 mm. .. The diameter of the inhibition zone of the NP‐AM/FK@OMV‐P/H coating was 21.7 ± 0.8 mm for ATCC 25 922 agar plates and 26.7 ± 1.5 mm for GIM1.457.

    Article Title: Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
    Article Snippet: The OMV nanoparticle had a higher action on E.coli GIM 1.457 compared with the ATCC 25 922 strain, and so the onset time at 0–6 h of inhibition was obviously shorter. .. The inhibition zone diameters of ATCC 25 922 agar plates coated with NP‐AM@OMV‐P/H and NP‐AM/FK@OMV‐P/H coatings were 13.5 ± 0.3, 15.0 ± 0.3, and GIM1.457 agar plates were 14.0 ± 0.8, 16.5 ± 0.5 mm, respectively (Figure ). ..

    Article Title: Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
    Article Snippet: As shown in Figure 3 A , the inhibition zone diameter of the NP‐AM@OMV‐P/H coating on ATCC 25 922 agar plates was 19.5 ± 0.5 mm, and that on GIM1.457 agar plates was 23.7 ± 1.5 mm. .. The diameter of the inhibition zone of the NP‐AM/FK@OMV‐P/H coating was 21.7 ± 0.8 mm for ATCC 25 922 agar plates and 26.7 ± 1.5 mm for GIM1.457. ..

    Article Title: Novel Nano Drug-Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI.
    Article Snippet: As shown in Figure 3A, the inhibition zone diameter of the NP-AM@OMV-P/H coating on ATCC 25 922 agar plates was 19.5 ± 0.5 mm, and that on GIM1.457 agar plates was 23.7 ± 1.5 mm. .. The diameter of the inhibition zone of the NP-AM/FK@OMV-P/H coating was 21.7 ± 0.8 mm for ATCC 25 922 agar plates and 26.7 ± 1.5 mm for GIM1.457. ..

    Article Title: Novel Nano Drug-Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI.
    Article Snippet: OMV drug-loaded nanoparticles were more enriched on the dominant strain E.coli GIM1.457 compared with the ATCC 25 922 strain (Figure S8), presumably owing to the sensitivity of EcN OMVs to the related bacterial strain. .. As shown in Figure 3A, the inhibition zone diameter of the NP-AM@OMV-P/H coating on ATCC 25 922 agar plates was 19.5 ± 0.5 mm, and that on GIM1.457 agar plates was 23.7 ± 1.5 mm. .. The diameter of the inhibition zone of the NP-AM/FK@OMV-P/H coating was 21.7 ± 0.8 mm for ATCC 25 922 agar plates and 26.7 ± 1.5 mm for GIM1.457.

    Bacteria:

    Article Title: Development of Stabilizing Solution for Long-Term Storage of Bacteriophages at Room Temperature and Application to Control Foodborne Pathogens
    Article Snippet: .. Next, 10 μL of each dilution was spotted onto overlayed agar plates containing host bacteria or a mixture of 100 μL of each dilution, 100 μL of the host bacteria overnight culture, and 0.4% TSB soft agar was poured onto agar plates ( E. coli NCTC 12079 for LEC1 and S. aureus ATCC 25923 for LSA5). ..

    Incubation:

    Article Title: Ursodeoxycholic acid inhibits biofilm formation and bacterial adhesion of Clostridioides difficile.
    Article Snippet: Ursodeoxycholic acid (UDCA) exhibits limited direct bacteriostatic activity against Clostridioides difficile with a high MIC90 of >128 μg/mL against 121 clinical strains.. However, compared with vancomycin, UDCA significantly impedes biofilm formation and bacterial adherence at subinhibitory concentrations, which may be the therapeutic advantages of UDCA and support this old drug to be further developed for CDI prevention.. Clostridioides difficile colonizes the large intestine and induces intestinal inflammation by adhering to the epithelial cells and producing toxins [1].



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    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on <t>Columbia</t> <t>blood</t> <t>agar</t> <t>plates</t> after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.
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    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on <t>Columbia</t> <t>blood</t> <t>agar</t> <t>plates</t> after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.
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    Image Search Results


    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.

    Journal: iScience

    Article Title: A tumor-resident Staphylococcus epidermidis strain exhibits genomic and metabolic traits driving hepatocellular carcinoma progression

    doi: 10.1016/j.isci.2026.116084

    Figure Lengend Snippet: Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.

    Article Snippet: Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains.

    Techniques: Isolation, Sequencing, Mass Spectrometry, Staining, Comparison, Incubation, Cell Culture, Inhibition